Anti-CIITA antibody (ab117598)
Key features and details
- Rabbit polyclonal to CIITA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CIITA antibody
See all CIITA primary antibodies -
Description
Rabbit polyclonal to CIITA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide from N terminal region of Human CIITA conjugated to KLH
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Positive control
- Human tonsil tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro). -
Involvement in disease
Bare lymphocyte syndrome 2 -
Sequence similarities
Contains 4 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Domain
The acetyltransferase domain is necessary for activation of both class I and class II transcription.
The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization. -
Post-translational
modificationsAutophosphorylated, affecting interaction with TAF7. -
Cellular localization
Nucleus. Nucleus, PML body. Recruited to PML body by PML. - Information by UniProt
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Database links
- Entrez Gene: 4261 Human
- Entrez Gene: 12265 Mouse
- Entrez Gene: 85483 Rat
- Omim: 600005 Human
- SwissProt: P33076 Human
- SwissProt: P79621 Mouse
- Unigene: 701991 Human
- Unigene: 249560 Mouse
see all -
Alternative names
- C2TA antibody
- C2TA_HUMAN antibody
- CIITA antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab117598 has been referenced in 2 publications.
- Sæterstad S et al. Profound gene expression changes in the epithelial monolayer of active ulcerative colitis and Crohn's disease. PLoS One 17:e0265189 (2022). PubMed: 35275975
- Zhu D et al. Major histocompatibility complexes are up-regulated in glomerular endothelial cells via activation of c-Jun N-terminal kinase in 5/6 nephrectomy mice. Br J Pharmacol 177:5131-5147 (2020). PubMed: 32830316