Overview

  • Product nameAnti-CIITA antibody
    See all CIITA primary antibodies
  • Description
    Rabbit polyclonal to CIITA
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-333 of Human CIITA

Properties

Applications

Our Abpromise guarantee covers the use of ab28349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 130 kDa (predicted molecular weight: 125 kDa).
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • FunctionEssential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • Involvement in diseaseBare lymphocyte syndrome 2
  • Sequence similaritiesContains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • DomainThe acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • Post-translational
    modifications
    Autophosphorylated, affecting interaction with TAF7.
  • Cellular localizationNucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

References for Anti-CIITA antibody (ab28349)

This product has been referenced in:
  • Casals C  et al. Lipopolysaccharide up-regulates MHC class II expression on dendritic cells through an AP-1 enhancer without affecting the levels of CIITA. J Immunol 178:6307-15 (2007). Mouse . Read more (PubMed: 17475859) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"