Overview

  • Product name
  • Description
    Rabbit polyclonal to CIITA
  • Specificity
    This antibody recognizes CIITA.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog
  • Immunogen

    A region within synthetic peptide: MALWESLQQQ GEAQLLQAAE EKFTIEPFKA KSPKDVEDLD RLVQTQRLRN, corresponding to amino acids 918-967 of Mouse CIITA

  • Positive control
    • NIH3T3 cell lysate
  • General notes


    Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Antibodies to this transactivator are useful in the study of diseases of pathological MHC class II expression.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 125 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Essential for transcriptional activity of the HLA class II promoter; activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. May act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter. Also mediates enhanced MHC class I transcription; the promoter element requirements for CIITA-mediated transcription are distinct from those of constitutive MHC class I transcription, and CIITA can functionally replace TAF1 at these genes. Exhibits intrinsic GTP-stimulated acetyltransferase activity. Exhibits serine/threonine protein kinase activity: can phosphorylate the TFIID component TAF7, the RAP74 subunit of the general transcription factor TFIIF, histone H2B at 'Ser-37' and other histones (in vitro).
  • Involvement in disease
    Bare lymphocyte syndrome 2
  • Sequence similarities
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • Domain
    The acetyltransferase domain is necessary for activation of both class I and class II transcription.
    The GTP-binding motif doesn't confer GTPase activity but promotes nuclear localization.
  • Post-translational
    modifications
    Autophosphorylated, affecting interaction with TAF7.
  • Cellular localization
    Nucleus. Nucleus, PML body. Recruited to PML body by PML.
  • Information by UniProt
  • Database links
  • Alternative names
    • C2TA antibody
    • C2TA_HUMAN antibody
    • CIITA antibody
    • CIITA IV antibody
    • Class II major histocompatibility complex transactivator antibody
    • Class II transactivator antibody
    • MHC class II transactivator antibody
    • MHC class II transactivator type III antibody
    • MHC2TA antibody
    • NLR family acid domain containing antibody
    • NLRA antibody
    • Nucleotide binding oligomerization domain leucine rich repeat and acid domain containing antibody
    see all

Images

  • Anti-CIITA antibody (ab49132) at 0.125 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 125 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Kitamura N  et al. Role of PU.1 in MHC class II expression through transcriptional regulation of class II transactivator pI in dendritic cells. J Allergy Clin Immunol 129:814-824.e6 (2012). Read more (PubMed: 22112519) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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