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Tissue/ cell preparation - BHK a21 cytoskeletal preparation.
This antibody may be used as a tool in the detection of ciliated cells in normal and pathalogical human specimens and for investigating mechanisms of ciliogenesis.
Our Abpromise guarantee covers the use of ab49852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 44-45 kDa.|
|ICC||Use a concentration of 1 - 2 µg/ml.|
|Electron Microscopy||Use at an assay dependent concentration.|
ab49852 staining Ciliated Cell Marker in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized in ice for 10 minutes and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA in PBST (PBS 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
ab49852 has not yet been referenced specifically in any publications.