Anti-Ciliated Cell Marker antibody [LhS28] (ab49852)

Overview

  • Product name
    Anti-Ciliated Cell Marker antibody [LhS28]
  • Description
    Mouse monoclonal [LhS28] to Ciliated Cell Marker
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC, Electron Microscopymore details
  • Species reactivity
    Reacts with: Hamster, Cow, Human
  • Immunogen

    Tissue/ cell preparation - BHK a21 cytoskeletal preparation.

  • Positive control
    • BHK a21 cells
  • General notes


    This antibody may be used as a tool in the detection of ciliated cells in normal and pathalogical human specimens and for investigating mechanisms of ciliogenesis.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    This antibody may be used as a tool in the detection of ciliated cells in normal and pathalogical human specimens and for investigating mechanisms of ciliogenesis.
  • Clonality
    Monoclonal
  • Clone number
    LhS28
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 44-45 kDa.
ICC Use a concentration of 1 - 2 µg/ml.
Electron Microscopy Use at an assay dependent concentration.

Target

  • Relevance
    This protein is expressed in the sub apical zone of cilliated epithelial cells of the Fallopian tube and upper respiratory tract, but not in ciliated ependyma, non ciliated epithelia or testis containing developing spermatozoa. This protein is associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays.

Anti-Ciliated Cell Marker antibody [LhS28] images

  • ab49852 staining Ciliated Cell Marker in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized in ice for 10 minutes and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA in PBST (PBS 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

References for Anti-Ciliated Cell Marker antibody [LhS28] (ab49852)

ab49852 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Cow Tissue sections (Oviduct)
Specification
Oviduct
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Jan 06 2014

Thank you very much for your interest in our antibodies.

Our antibody ab49852 is suitable for use as a ciliated cell marker, but has not been validated for use in Flow Cytometry. This antibody also contains Sodium Azide, which would not be s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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