Anti-Cingulin antibody (ab117778)

Overview

  • Product nameAnti-Cingulin antibody
    See all Cingulin primary antibodies
  • Description
    Rabbit polyclonal to Cingulin
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 350-400 of Human Cingulin (NP_065821.1).

  • Positive control
    • Jurkat, HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab117778 was affinity purified using an epitope specific to Cingulin immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionProbably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
  • Tissue specificityLocalized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
  • Sequence similaritiesBelongs to the cingulin family.
  • DomainDeletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
  • Cellular localizationCell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6330408J11Rik antibody
    • AI647528 antibody
    • AI987749 antibody
    • cgn antibody
    • CING_HUMAN antibody
    • Cingulin antibody
    • DKFZp779N1112 antibody
    • FLJ39281 antibody
    • KIAA1319 antibody
    • MGC118157 antibody
    see all

Anti-Cingulin antibody images

  • All lanes : Anti-Cingulin antibody (ab117778) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : Jurkat whole cell lysates at 50 µg
    Lane 4 : HeLa whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 136 kDa


    Exposure time : 3 minutes
  • Detection of Cingulin by Western Blot of Immunprecipitate.
    ab117778 at 1µg/ml staining Cingulin in 293T whole cell lysate immunoprecipitated using ab117778 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-Cingulin antibody (ab117778)

ab117778 has not yet been referenced specifically in any publications.

Product Wall

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