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Rabbit polyclonal to Cingulin
Predicted to work with:
Synthetic peptide, corresponding to a region between amino acids 200-250 of Human Cingulin (NP_065821.1).
293T, Jurkat, and HeLa whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7-8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
Use at 2-10 µg/mg of lysate.
Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.
Belongs to the cingulin family.
Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.
Cell junction > tight junction. Localizes to the apical junction complex composed of tight and adherens junctions.
Information by UniProt
Western blot - Anti-Cingulin antibody (ab117796)
All lanes : Anti-Cingulin antibody (ab117796) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg/ml Lane 2 : 293T whole cell lysate at 15 µg/ml Lane 3 : Jurkat whole cell lysate at 50 µg/ml Lane 4 : HeLa whole cell lysate at 50 µg/ml Developed using the ECL technique. Predicted band size: 136 kDa Exposure time: 10 seconds
Immunoprecipitation - Anti-Cingulin antibody (ab117796)
ab117796 at 1 µg/ml staining Cingulin in 293T cell lysate immunoprecipitated using ab117796 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds
has not yet been referenced specifically in any publications.
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