The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 24 kDa.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling.
Belongs to the CINP family.
Phosphorylated by CDC7 but not by CDK2.
Nucleus. Binds to nuclear under G1 conditions, and dissociates from chromatin with the start of DNA replication.
Cyclin dependent kinase 2 interacting protein antibody
Cyclin-dependent kinase 2-interacting protein antibody
Western blot - Anti-CINP antibody (ab176181)
Anti-CINP antibody (ab176181) at 1/1000 dilution + CEM cell lysate at 35 µg
Predicted band size : 24 kDa
Flow Cytometry - Anti-CINP antibody (ab176181)
Flow cytometric analysis of CEM cells labeling CINP with ab176181 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-Rabbit secondary antibodies were used for the analysis.