Anti-Cip4 antibody (ab72220)
Key features and details
- Rabbit polyclonal to Cip4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cip4 antibody
See all Cip4 primary antibodies -
Description
Rabbit polyclonal to Cip4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
A region between residues 551 and 601 of human Cip4
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Positive control
- HeLa cytoplasmic or membrane lysate 293T cytoplasmic or membrane lysate Mouse NIH3T3 cytoplasmic or membrane lysate
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72220 was affinity purified using an epitope specific to Cip4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL. -
Tissue specificity
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. -
Sequence similarities
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
Post-translational
modificationsTyrosine phosphorylated. Also phosphorylated by PKA. -
Cellular localization
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. - Information by UniProt
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Database links
- Entrez Gene: 9322 Human
- Entrez Gene: 106628 Mouse
- Entrez Gene: 100172170 Orangutan
- Entrez Gene: 116717 Rat
- Omim: 604504 Human
- SwissProt: Q15642 Human
- SwissProt: Q8CJ53 Mouse
- SwissProt: Q5RCJ1 Orangutan
see all -
Alternative names
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
see all
Images
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All lanes : Anti-Cip4 antibody (ab72220) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Detection of Human Cip4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with an antibody recognising an upstream epitope in lane 1, ab72220 at 3 µg in lane 2 and control IgG in lane 3. Image shows immunoprecipitated Cip4 detected using post IP WB, loading 20% of IP and using ab72220 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72220 has been referenced in 1 publication.
- Zhu YC et al. Suppression of CIP4/Par6 attenuates TGF-ß1-induced epithelial-mesenchymal transition in NRK-52E cells. Int J Mol Med 40:1165-1171 (2017). PubMed: 28848997