Overview

  • Product nameAnti-CIRBP antibody
    See all CIRBP primary antibodies
  • Description
    Goat polyclonal to CIRBP
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-QAGKSSDNRSR

    , corresponding to internal sequence amino acids 81-91 of Human CIRBP (NP_001271.1).

  • Positive control
    • Mouse and Rat Testis lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab106239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).

Target

  • FunctionCold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • DomainBoth the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • Post-translational
    modifications
    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • Cellular localizationNucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Anti-CIRBP antibody images

  • Anti-CIRBP antibody (ab106239) at 0.05 µg/ml + Mouse Testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 19 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References for Anti-CIRBP antibody (ab106239)

ab106239 has not yet been referenced specifically in any publications.

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