Anti-CIRBP antibody - C-terminal (ab166775)

Overview

  • Product name
    Anti-CIRBP antibody - C-terminal
    See all CIRBP primary antibodies
  • Description
    Goat polyclonal to CIRBP - C-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster, Cow, Dog, Turkey, Pig, Monkey, Opossum, Common marmoset, Orangutan, Platypus
  • Immunogen

    Synthetic peptide C-

    RDSYDSYATHNE

    corresponding to C terminal amino acids 161-172 of Human CIRBP (NP_001271.1).

  • Positive control
    • Human Testis tissue; Mouse and Rat testis lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression.
  • Post-translational
    modifications
    Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
    Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
  • Cellular localization
    Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins.
  • Information by UniProt
  • Database links
  • Alternative names
    • A18 hnRNP antibody
    • A18HNRNP antibody
    • cirbp antibody
    • CIRBP_HUMAN antibody
    • CIRP antibody
    • Cold inducible RNA binding protein antibody
    • Cold-inducible RNA-binding protein antibody
    • Glycine rich RNA binding protein antibody
    • Glycine-rich RNA-binding protein CIRP antibody
    • testicular tissue protein Li 39 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling CIRBP with ab166775 at 3.75 µg/ml.
  • Anti-CIRBP antibody - C-terminal (ab166775) at 0.01 µg/ml + Mouse testis lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa



    Primary incubation: 1 hour.

References

ab166775 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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