Overview

  • Product nameAnti-CISD1 antibody [2B3]
    See all CISD1 primary antibodies
  • Description
    Mouse monoclonal [2B3] to CISD1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human CISD1 produced in HEK293T cells (NP_060934).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY CISD1; Jurkat cells.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab118027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 12 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionPlays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.
  • Tissue specificityExpression is reduced in cells derived from cystic fibrosis patients.
  • Sequence similaritiesBelongs to the CISD protein family.
  • Cellular localizationMitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU043990 antibody
    • AW743335 antibody
    • C10orf70 antibody
    • CDGSH iron sulfur domain 1 antibody
    • CDGSH iron-sulfur domain-containing protein 1 antibody
    • CISD 1 antibody
    • CISD1 antibody
    • CISD1_HUMAN antibody
    • D10Ertd214e antibody
    • MDS029 antibody
    • MGC14684 antibody
    • MitoNEET antibody
    • RGD1309529 antibody
    • ZCD1 antibody
    • Zinc finger CDGSH type domain 1 antibody
    see all

Anti-CISD1 antibody [2B3] images

  • All lanes : Anti-CISD1 antibody [2B3] (ab118027) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CISD1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 12 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203308)
  • ab118027, at 1/100 dilution, staining CISD1 in COS7 cells transiently transfected by pCMV6-ENTRY CISD1 by Immunofluorescence.
  • Flow cytometric Analysis of CISD1 staining in Jurkat cells, using ab118027 at 1/100 dilution(Red), compared to a nonspecific negative control antibody(Blue).

References for Anti-CISD1 antibody [2B3] (ab118027)

This product has been referenced in:
  • Salem AF  et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 11:4174-80 (2012). Read more (PubMed: 23070475) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"