Overview

  • Product name
  • Description
    Rabbit polyclonal to CISD2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: VLESVARIVK VQLPAYLKRL PVPESITGFA RLTVSEWLRL LPFLGVLALL , corresponding to N terminal amino acids 1-50 of Human CISD2 (NP_001008389)

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Regulator of autophagy that contributes to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. Participates in the interaction of BCL2 with BECN1 and is required for BCL2-mediated depression of endoplasmic reticulum Ca(2+) stores during autophagy. Contributes to BIK-initiated autophagy, while it is not involved in BIK-dependent activation of caspases. Involved in life span control, probably via its function as regulator of autophagy.
  • Tissue specificity
    Testis, small intestine, kidney, lung, brain, heart, pancreas and platelets.
  • Involvement in disease
    Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2) [MIM:604928]. A rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. Other manifestations include diabetes insipidus, sensorineural deafness, dementia, psychiatric illnesses. WFS2 patients additionally show a strong bleeding tendency and gastrointestinal ulceration. Diabetes insipidus may be absent.
  • Sequence similarities
    Belongs to the CISD protein family. CISD2 subfamily.
  • Cellular localization
    Endoplasmic reticulum membrane. Mitochondrion outer membrane. According to PubMed:20010695, it mainly localizes to the endoplasmic reticulum. However, experiments in mouse showed that it mainly localizes to the mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500009M05Rik antibody
    • 1500026J14Rik antibody
    • 1500031D15Rik antibody
    • AI848398 antibody
    • B630006A20Rik antibody
    • CDGSH iron sulfur domain 2 antibody
    • CDGSH iron-sulfur domain-containing protein 2 antibody
    • CDGSH type domain 2 antibody
    • CISD2 antibody
    • CISD2_HUMAN antibody
    • Endoplasmic reticulum intermembrane small protein antibody
    • ERIS antibody
    • Miner1 antibody
    • MitoNEET related 1 antibody
    • MitoNEET-related 1 protein antibody
    • NAF-1 antibody
    • Noxp70 antibody
    • Nutrient deprivation autophagy factor 1 antibody
    • Nutrient-deprivation autophagy factor-1 antibody
    • OTTHUMP00000219576 antibody
    • RGD1566242 antibody
    • WFS2 antibody
    • Zcd2 antibody
    • Zinc finger antibody
    • Zinc finger, CDGSH type domain 2 antibody
    see all

Images

  • Anti-CISD2 antibody (ab80509) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 15 kDa
    Observed band size : 15 kDa

References

ab80509 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80509.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up