Overview

  • Product nameAnti-CISH antibody
    See all CISH primary antibodies
  • Description
    Rabbit polyclonal to CISH
  • Tested applicationsSuitable for: ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 170-185 of CIS-1

  • Positive control
    • Mouse embryo lysate.
  • General notes


    This product is raised against Cis-1.

Properties

Applications

Our Abpromise guarantee covers the use of ab5923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 47 kDa.
IP Use 3-5µg for 107 cells.

Target

  • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificityExpressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Post-translational
    modifications
    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • Database links
  • Alternative names
    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all

References for Anti-CISH antibody (ab5923)

ab5923 has not yet been referenced specifically in any publications.

Product Wall

The antibody has been tested in IP against endogenous protein, not recombinant protein. It was raised against a synthetic peptide corresponding to 170-185 amino acids of human/mouse/rat CIS-1 therefore if your recombinant protein of interest covers thi...

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Thank you for your enquiry and patience. I have just heard back from the originator of this antibody regarding your question. Ab5923 was tested in IP using mouse embryonic cells. The IP protocol that they followed is below: - 2 ul cell lysate -add ...

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As I explained to you on the phone, there was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I apologize for this mistake and the datasheet has been updated to reflect this information. I can of course give you...

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Thank you for your patience. As ab5923 was originated outside Abcam I had to contact the originator regarding your question and have just heard back. There was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I ap...

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Thank you for your enquiry and your interest in our products. We would like to confirm that these two antibodies (ab3691 and ab5923) have been characterized for ELISA application. However, they have not been tested together in a sandwich ELISA system.

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Thank you for your patience, I have only just heard back from the originator of ab5923. Ab5923 is anti-CIS which recognizes the band at 47 kDa. Ab3691 recognizes the 24 kDa immunoreactive protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"