Overview

  • Product nameAnti-CISH antibody
    See all CISH primary antibodies
  • Description
    Mouse polyclonal to CISH
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human CISH protein (NP_659508.1)

  • Positive control
    • Human spleen lysate. CISH transfected 293T cell lysate. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificityExpressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Post-translational
    modifications
    Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
  • Information by UniProt
  • Database links
  • Alternative names
    • BACTS2 antibody
    • cis 1 antibody
    • CIS antibody
    • CIS-1 antibody
    • CIS1 antibody
    • Cish antibody
    • CISH_HUMAN antibody
    • Cytokine inducible inhibitor of signaling type 1B antibody
    • Cytokine inducible SH2 containing protein antibody
    • Cytokine-inducible SH2-containing protein antibody
    • G18 antibody
    • Protein G18 antibody
    • SOCS antibody
    • Suppressor of cytokine signaling antibody
    see all

Anti-CISH antibody images

  • Anti-CISH antibody (ab88383) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 29 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CISH antibody (ab88383) at 1 µg/ml

    Lane 1 : CISH transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of CISH in HeLa cells using ab88383 at 10µg/ml.

References for Anti-CISH antibody (ab88383)

ab88383 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

I am sorry to hear that the antibody is not working as previously.

As we discussed over the phone, I have arranged for a free of charge replacement with a new lot number (GR89220-1) which you will receiv...

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Thank you for contacting us.

I checked, but we did not unpublish any CISH antibody in the last couple years.
Also, I did not see any changes made to the MW. Our internal notes do not indicate that.

The predicted MWfor human CI...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"