Anti-CIT antibody (ab86782)
Key features and details
- Rabbit polyclonal to CIT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CIT antibody
See all CIT primary antibodies -
Description
Rabbit polyclonal to CIT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to Human CIT aa 1900-2000.
Database link: O14578 -
Positive control
- HeLa whole cell lysate (ab150035) 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 231 kDa.
Or 0.1 - 1µg/ml. |
|
IP |
Use at 10 µg/mg of lysate.
|
Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 231 kDa. Or 0.1 - 1µg/ml. |
IP
Use at 10 µg/mg of lysate. |
Target
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Function
Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11113 Human
- Omim: 605629 Human
- SwissProt: O14578 Human
- Unigene: 119594 Human
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Alternative names
- CIT antibody
- Citron (rho interacting serine/threonine kinase 21) antibody
- Citron antibody
see all
Images
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All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 231 kDa
Exposure time: 30 seconds -
1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab86782 has been referenced in 3 publications.
- Lavado-García J et al. Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV-1 virus-like particles. Biotechnol Bioeng 118:1649-1663 (2021). PubMed: 33463716
- Liu Z et al. Novel circRNA_0071196/miRNA-19b-3p/CIT axis is associated with proliferation and migration of bladder cancer. Int J Oncol 57:767-779 (2020). PubMed: 32705161
- Sgrò F et al. Tissue-specific control of midbody microtubule stability by Citron kinase through modulation of TUBB3 phosphorylation. Cell Death Differ 23:801-13 (2016). PubMed: 26586574