Overview

  • Product name
  • Description
    Rabbit polyclonal to CIT
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide, from a region within amino acids 1925-1975 of Human CIT (NP_009105.1)

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 10µg/mg of lysate.
    WB: 1/2000 - 1/10000 (or 0.1 - 1µg/ml). Predicted molecular weight: 231 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      CIT is a putative RHO/RAC effector that binds to the GTP bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. This protein exhibits dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system.
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • Citron (rho interacting serine/threonine kinase 21) antibody
      • Citron antibody
      • Citron Rho interacting kinase antibody
      • CRIK antibody
      • EC 2.7.11.1 antibody
      • KIAA0949 antibody
      • Rho interacting serine/threonine kinase 21 antibody
      • Rho interacting serine/threonine protein kinase 21 antibody
      • serine/threonine-protein kinase 21 antibody
      • STK2 antibody
      • STK21 antibody
      see all

    Images

    • All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 231 kDa


      Exposure time : 30 seconds
    • 1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.

    References

    ab86782 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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