Overview

  • Product nameAnti-CIT antibody
    See all CIT primary antibodies
  • Description
    Rabbit polyclonal to CIT
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, from a region within amino acids 1925-1975 of Human CIT (NP_009105.1)

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 10µg/mg of lysate.
    WB: 1/2000 - 1/10000 (or 0.1 - 1µg/ml). Predicted molecular weight: 231 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCIT is a putative RHO/RAC effector that binds to the GTP bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. This protein exhibits dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Citron (rho interacting serine/threonine kinase 21) antibody
      • Citron antibody
      • Citron Rho interacting kinase antibody
      • CRIK antibody
      • EC 2.7.11.1 antibody
      • KIAA0949 antibody
      • Rho interacting serine/threonine kinase 21 antibody
      • Rho interacting serine/threonine protein kinase 21 antibody
      • serine/threonine-protein kinase 21 antibody
      • STK2 antibody
      • STK21 antibody
      see all

    Anti-CIT antibody images

    • All lanes : Anti-CIT antibody (ab86782) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 231 kDa


      Exposure time : 30 seconds
    • 1 mg HeLa whole cell lysate was immunoprecipitated with 10 µg ab86782 (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was loaded onto a Western blot and labelled with ab86782 at 1 µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds.

    References for Anti-CIT antibody (ab86782)

    ab86782 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86782.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"