Overview

  • Product name
  • Description
    Mouse monoclonal to CITED1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: SMQLQKLNSQ YQGMAAATPG QPGEAGPLQN WDFGAQAGGA ESLSPSAGAQ SPAIIDSDPV DEEVLMSLVV ELGLDRANEL PELWLGQNEF DFTADFPSSC , corresponding to amino acids 94-194 of Human CITED1

  • Positive control
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab213429 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab55467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner.
    • Tissue specificity
      Expressed only in melanocytes and testis.
    • Sequence similarities
      Belongs to the CITED family.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional coactivator activity.
    • Cellular localization
      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABCC1 antibody
      • AI316840 antibody
      • AU019144 antibody
      • Cbp/p300-interacting transactivator 1 antibody
      • Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1 antibody
      • Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 antibody
      • CITE1_HUMAN antibody
      • CITED1 antibody
      • melanocyte specific gene 1 antibody
      • Melanocyte specific protein 1 antibody
      • Melanocyte-specific protein 1 antibody
      • MSG1 antibody
      see all

    Images



    • Predicted band size : 20 kDa
      CITED1 antibody (ab55467) at 1ug/lane + A-431 cell lysate at 25ug/lane.

    References

    ab55467 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue (Kidney Tissue)
    Specification
    Kidney Tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Tracy Tran

    Verified customer

    Submitted Feb 10 2016

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (human kidney cells)
    Specification
    human kidney cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 18 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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