Overview

  • Product name
  • Description
    Mouse monoclonal to CITED1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment: SMQLQKLNSQ YQGMAAATPG QPGEAGPLQN WDFGAQAGGA ESLSPSAGAQ SPAIIDSDPV DEEVLMSLVV ELGLDRANEL PELWLGQNEF DFTADFPSSC , corresponding to amino acids 94-194 of Human CITED1 with proprietary tag. (NP_004134)

  • Positive control
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab87978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1.2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently.

Target

  • Function
    Transcriptional coactivator of the p300/CBP-mediated trancription complex. Enhances SMAD-mediated transcription by strengthening the functional link between the DNA-binding SMAD transcription factors and the p300/CBP transcription coactivator complex. Stimulates estrogen-dependent transactivation activity mediated by estrogen receptors signaling; stabilizes the interaction of estrogen receptor ESR1 and histone acetyltransferase EP300. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Induces transcription from estrogen-responsive promoters and protection against cell death. Potentiates EGR2-mediated transcriptional activation activity from the ERBB2 promoter. Acts as an inhibitor of osteoblastic mineralization through a cAMP-dependent parathyroid hormone receptor signaling. May play a role in pigmentation of melanocytes. Associates with chromatin to the estrogen-responsive TGF-alpha promoter region in a estrogen-dependent manner.
  • Tissue specificity
    Expressed only in melanocytes and testis.
  • Sequence similarities
    Belongs to the CITED family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation changes in a cell cycle-dependent manner and reduces its transcriptional coactivator activity.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm by a nuclear export signal and (NES) in a CRM1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCC1 antibody
    • AI316840 antibody
    • AU019144 antibody
    • Cbp/p300-interacting transactivator 1 antibody
    • Cbp/p300-interacting transactivator with GLU/ASP-rich C-terminal domain, 1 antibody
    • Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1 antibody
    • CITE1_HUMAN antibody
    • CITED1 antibody
    • melanocyte specific gene 1 antibody
    • Melanocyte specific protein 1 antibody
    • Melanocyte-specific protein 1 antibody
    • MSG1 antibody
    see all

Images

  • Anti-CITED1 antibody (ab87978) at 5 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CITED1 antibody (ab87978) at 1 µg/ml

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
  • Immunoperoxidase of monoclonal antibody ab87978 at 1.2 ug/ml to CITED1 on formalin-fixed paraffin-embedded Human testis.
  • Capture ELISA using recombinant protein. Detection limit is approximately 0.03ng/ml as a capture antibody.
  • Anti-CITED1 antibody (ab87978) at 5 µg/ml + Recombinant protein used as immunogen at 0.2 µg/ml

    Predicted band size : 20 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Vargas D  et al. Modifications of Human Subcutaneous ADMSC after PPAR? Activation and Cold Exposition. Stem Cells Int 2015:196348 (2015). WB . Read more (PubMed: 26339249) »
  • Sehic D  et al. Evaluation of CITED1, SIX1, and CD56 protein expression for identification of blastemal elements in Wilms tumor. Am J Clin Pathol 141:828-33 (2014). Read more (PubMed: 24838327) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (kidney)
Specification
kidney
Permeabilization
Yes - Methanol
Fixative
Paraformaldehyde
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Verified customer

Submitted Dec 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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