Overview

  • Product name
    Anti-CITED2 antibody [EPR3416(2)]
    See all CITED2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3416(2)] to CITED2
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CITED2 aa 200 to the C-terminus. The exact sequence is proprietary.

  • Positive control
    • WB: Saos-2, U937, NIH/3T3 and HeLa cell lysates.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
    • Sequence similarities
      Belongs to the CITED family.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ASD8 antibody
      • Cbp/p300 interacting transactivator 2 antibody
      • Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2 antibody
      • Cbp/p300-interacting transactivator 2 antibody
      • CITE2_HUMAN antibody
      • CITED 2 antibody
      • CITED2 antibody
      • melanocyte-specific gene 1-related gene 1 antibody
      • MRG 1 antibody
      • MRG-1 antibody
      • MRG1 antibody
      • MSG related protein 1 antibody
      • MSG-related protein 1 antibody
      • MSG1-related gene 1 antibody
      • P35srj antibody
      • VSD2 antibody
      see all

    Anti-CITED2 antibody [EPR3416(2)] images

    • Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/2000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 20 µg (purified) + Saos-2 whole cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution (purified) + U937 whole cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution (unpurified)

      Lane 1 : Saos-2 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa

    References for Anti-CITED2 antibody [EPR3416(2)] (ab108345)

    This product has been referenced in:
    • Sakai M  et al. The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch. Nat Commun 7:13147 (2016). Mouse . Read more (PubMed: 27874008) »
    • Fame RM  et al. Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity. J Neurosci 36:6403-19 (2016). Read more (PubMed: 27307230) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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