Overview

  • Product nameAnti-CITED4 antibody
    See all CITED4 primary antibodies
  • Description
    Rabbit polyclonal to CITED4
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    A region within synthetic peptide: MDSGLRPRGA PLGPPPPPGT LAYGSFGSPV SFQPFPVSQS PGAGSTHLQS, corresponding to N terminal amino acids 37-86 of Mouse CITED4

  • Positive control
    • SP2/0 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab48838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionActs as transcriptional coactivator for TFAP2/AP-2. Enhances estrogen-dependent transactivation mediated by estrogen receptors. May function as an inhibitor of transactivation by HIF1A by disrupting HIF1A interaction with CREBBP. May be involved in regulation of gene expression during development and differentiation of blood cells, endothelial cells and mammary epithelial cells.
  • Tissue specificityExpressed in most tissues examined with highest levels of expression in heart, liver, skeletal muscle and pancreas. Also expressed in bladder cell line ECV304 and in various breast cancer cell lines. Also detected in both in situ and invasive breast tumors where its expression is down-regulated and mostly restricted to the cytoplasm of malignant epithelium. Down-regulation of expression is associated with elevated levels of HIF1A and increased tumor growth and angiogenesis.
  • Sequence similaritiesBelongs to the CITED family.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU019445 antibody
    • AW742964 antibody
    • Cbp/p300 interacting transactivator 4 antibody
    • cbp/p300 interacting transactivator, with glu/asp rich carboxy terminal domain, 4 antibody
    • Cbp/p300-interacting transactivator 4 antibody
    • CITE4_HUMAN antibody
    • Cited4 antibody
    • MRG 2 antibody
    • MRG-2 antibody
    • Mrg2 antibody
    • MSG1 related protein 2 antibody
    • MSG1-related protein 2 antibody
    • Transcriptional co-activator 4 antibody
    see all

Anti-CITED4 antibody images

  • Anti-CITED4 antibody (ab48838) at 1.25 µg/ml + Sp2/0 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 20 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-CITED4 antibody (ab48838)

ab48838 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"