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Citrate Synthase Activity Assay Kit (ab119692) is used to determine mitochondrial citrate synthase (CS) activity in a sample at an immunocapture based manner. The enzyme is captured within the wells of the microplate, and activity is determined by recording color development of TNB, which is generated from DTNB present in the reaction of citrate synthesis. The overall reation product, TNB, absorbs at OD = 412 nm.
This kit specifically immunocaptures only native citrate synthase in well from the applied test sample. The immunocapture based activity assay allows researchers to measure citrate synthase activity with simple sample preparation and no need for mitochondria isolation.
This kit contains all of the required reagents for a fast and simple measurement of citrate synthase activity in a whole cell extract or in tissue homogenate.
Citrate synthase (CS, O75390) is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme is a 51.7 kDa enzyme that catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate (EC 220.127.116.11). This enzyme is an exclusive marker of the mitochondrial matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Storage: All components are shipped cold. 10X Reaction Reagent mixture is shipped lyophilized. Before use, rehydrate the reaction mixture by adding 1mL base buffer. Vortex the tube thoroughly to dissolve the lyophilized powder to prepare 10X Reaction solution. After hydration unused amounts of the 10X Reaction solution should be stored at -80°C for 6 months. Store all other components at 4°C. This kit is stable for 6 months from receipt.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X Reaction Reagent||1 vial|
|1X Base Buffer||1 x 25ml|
|20X Buffer||1 x 20ml|
|CS Microplate||1 unit|
|Extraction Buffer||1 x 15ml|
Our Abpromise guarantee covers the use of ab119692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|