Overview

  • Product nameAnti-Citrate synthetase antibody
    See all Citrate synthetase primary antibodies
  • Description
    Rabbit polyclonal to Citrate synthetase
  • Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Cow, Pig, Xenopus laevis
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 44-316 of Human Citrate synthetase (NP_938083).

  • Positive control
    • WB: 293T, A431, HeLa and HepG2 whole cell lysates and zebrafish skeletal muscle tissue and mouse liver mitochondrial homogenates. ICC/IF: HeLa and U2OS cells. IHC-P: Human colon, mouse intestine, mouse muscle and NCI-N87 xenograft tissues. IP: 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.

Target

Anti-Citrate synthetase antibody images

  • All lanes : Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa

    10% SDS-PAGE.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/200. Calls were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.

  • IHC image of ab96600 staining in Human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunoprecipitation of Citrate synthetase from 293T whole cell lysate using 5 μg of ab96600.

    Western blot analysis was performed using ab96600.

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/500. Calls were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of NCI-N87 xenograft tissue labelling Citrate synthetase with ab96600 at a dilution of 1/500.

  • All lanes : Anti-Citrate synthetase antibody (ab96600) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish liver homogenate (20ug)
    Lane 3 : Zebrafish skeletal muscle homogenate (20ug)
    Lane 4 : Mouse liver mitochondrial homogenate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

References for Anti-Citrate synthetase antibody (ab96600)

This product has been referenced in:
  • Fiuza-Luces C  et al. Muscle Signaling in Exercise Intolerance: Insights from the McArdle Mouse Model. Med Sci Sports Exerc N/A:N/A (2016). WB . Read more (PubMed: 27031745) »
  • Schiffer TA  et al. Control of human energy expenditure by cytochrome c oxidase subunit IV-2. Am J Physiol Cell Physiol 311:C452-61 (2016). IHC-Fr ; Human . Read more (PubMed: 27486093) »

See all 17 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MRC5 normal lung fibroblasts)
Specification MRC5 normal lung fibroblasts
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 13 2015

Application Western blot
Loading amount 0.5 µg
Gel Running Conditions Reduced Denaturing (4-15% Tris-glycine)
Sample Pig Recombinant protein (Citrate Synthase from porcine heart (Sigma))
Specification Citrate Synthase from porcine heart (Sigma)
Blocking step Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. Rachel Loh

Verified customer

Submitted Jan 15 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10% 37.5:1)
Sample Pig Tissue lysate - other (Skeletal muscle mitochondria purified)
Specification Skeletal muscle mitochondria purified
Blocking step 3% Casein in PBS with 0.1% Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing (10% 37.5:1)
Sample Cow Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step 3% Casein in PBS with 0.1% Tween-20 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

Submitted Oct 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ilan Sela

Verified customer

Submitted Aug 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 20 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 26 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (L6 myotubes)
Loading amount 20 µg
Specification L6 myotubes
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"