Overview

  • Product name
    Anti-Citrobacter koseri antibody
  • Description
    Rabbit polyclonal to Citrobacter koseri
  • Specificity
    This antibody targets Citrobacter koseri proteins.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with Citrobacter koseri. Not yet tested in other species.
  • Immunogen

    Full length native protein (purified) corresponding to Citrobacter koseri. Citrobacter koseri total proteins

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, ddH20
  • Concentration information loading...
  • Purification notes
    Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Citrobacter is a genus of gram-negative bacteria in the family of the Enterobacteriaceae. The species C. amalonaticus, C. koseri, and C. freundii use solely citrate as a carbon source. These bacteria can be found almost everywhere in soil, water, wastewater, etc. It can also be found in the human intestine. They are rarely the source of illnesses, except for infections of the urinary tract and infant meningitis.

Anti-Citrobacter koseri antibody images

  • ab37056 staining Citobacter koseri in mouse colon tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were snap frozen without fixation and incubated in 1% PFA in PBS for 10 minutes after cutting sections (5µm). Sections were permeabilized with 0.5% NP40 and blocked with 10% goat serum for 1 hour at 25°C. Sections were incubated with primary antibody (1/500 in PBS+ 5% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Citrobacter koseri antibody (ab37056)

This product has been referenced in:
  • Singh K  et al. The L-Arginine Transporter Solute Carrier Family 7 Member 2 Mediates the Immunopathogenesis of Attaching and Effacing Bacteria. PLoS Pathog 12:e1005984 (2016). ICC/IF . Read more (PubMed: 27783672) »

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (mouse colon sections (snap frozen))
Specification
mouse colon sections (snap frozen)
Permeabilization
Yes - 0.5% NP40
Fixative
snap frozen without fixation 1% PFA in PBS for 10min after sections cut
Username

Dr. Jerry Turner

Verified customer

Submitted Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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