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|Arginine-G-BSA, Glutamine-G-BSA, Ornithine-G-BSA||1 > 100,000|
|Conjuaged Citrulline and conjugated Homo-citrulline||1:1000|
Chemical/ Small Molecule by a Glutaraldehyde linker.
Our Abpromise guarantee covers the use of ab6464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/5000 - 1/10000.|
|IHC-Fr||1/1000 - 1/5000.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of breast DCIS tumor with apoptotic center labeling Citrulline with ab6464 at 1/100 dilution. Tissue was fixed in 10% buffered formalin for 24 hrs then transferred to 70% ethanol until processed and embedded and permeabilized with Tris Buffered Saline with 0.025% Triton X-100. Heat mediated antigen retrieval was performed using 10mM Sodium Citrate Buffer with 0.05% Tween 20 pH 6.0. Tissue was blocked with TBS with 10% normal (secondary species) serum and 1% BSA for 1 hour at 37°C, followed by incubation with Anti-Citrulline antibody (ab6464) in TBS with 1% BSA for 18 hours at 4°C at 1/100 dilution. A polyclonal goat anti rabbit IgG biotin conjugate secondary antibody was used at 1/200 dilution.