Overview

  • Product name
    Anti-CK1 epsilon antibody
    See all CK1 epsilon primary antibodies
  • Description
    Rabbit polyclonal to CK1 epsilon
  • Host species
    Rabbit
  • Specificity
    ab70110 detects endogenous levels of total CK1 epsilon protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human CK1 epsilon

  • Positive control
    • LOVO cell extracts, HeLa cells and human placenta tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70110 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificity
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Casein kinase 1 epsilon antibody
      • Casein kinase I antibody
      • Casein kinase I epsilon antibody
      • Casein kinase I isoform epsilon antibody
      • CKI epsilon antibody
      • CKI-epsilon antibody
      • CKIe antibody
      • CKIepsilon antibody
      • CSNK 1E antibody
      • CSNK1E antibody
      • DBT antibody
      • Doubletime, Drosophila, homolog of antibody
      • EC 2.7.11.1 antibody
      • epsilon isoform antibody
      • HCK IE antibody
      • KC1E antibody
      • KC1E_HUMAN antibody
      • kinase CK1 epsilon antibody
      • MGC 10398 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - CK1 epsilon antibody (ab70110)
      Immunocytochemistry/ Immunofluorescence - CK1 epsilon antibody (ab70110)
      ab70110 at 1/500 dilution staining CK1 epsilon in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CK1 epsilon antibody (ab70110)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CK1 epsilon antibody (ab70110)
      ab70110 at 1/50 dilution staining CK1 epsilon in human placenta by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
    • Western blot - CK1 epsilon antibody (ab70110)
      Western blot - CK1 epsilon antibody (ab70110)
      All lanes : Anti-CK1 epsilon antibody (ab70110) at 1/500 dilution

      Lane 1 : LOVO cell extracts
      Lane 2 : LOVO cell extracts with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    References

    This product has been referenced in:
    • Lopez-Guerra JL  et al. High casein kinase 1 epsilon levels are correlated with better prognosis in subsets of patients with breast cancer. Oncotarget 6:30343-56 (2015). IHC . Read more (PubMed: 26327509) »
    • Scoma HD  et al. The De-Ubiquitinylating Enzyme, USP2, Is Associated with the Circadian Clockwork and Regulates Its Sensitivity to Light. PLoS One 6:e25382 (2011). WB ; Human . Read more (PubMed: 21966515) »

    See all 2 Publications for this product

    Publishing research using ab70110? Please let us know so that we can cite the reference in this datasheet.

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