Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687)

Overview

  • Product name
    Anti-CK1 epsilon antibody - Carboxyterminal end
    See all CK1 epsilon primary antibodies
  • Description
    Rabbit polyclonal to CK1 epsilon - Carboxyterminal end
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human CK1 epsilon

  • Positive control
    • SKBR3 cell lysate, human breast carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function
    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificity
    Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

Anti-CK1 epsilon antibody - Carboxyterminal end images

  • Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687) at 1/100 dilution + SKBR3 cell lysate at 12.5 µg

    Secondary
    HRP anti rabbit

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 100 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Formalin fixed and paraffin embedded human breast carcinoma tissue reacted with 1/50 ab71687. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

References for Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687)

ab71687 has not yet been referenced specifically in any publications.

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