Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687)

Overview

  • Product nameAnti-CK1 epsilon antibody - Carboxyterminal end
    See all CK1 epsilon primary antibodies
  • Description
    Rabbit polyclonal to CK1 epsilon - Carboxyterminal end
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human CK1 epsilon

  • Positive control
    • SKBR3 cell lysate, human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Casein kinase 1 epsilon antibody
    • Casein kinase I antibody
    • Casein kinase I epsilon antibody
    • Casein kinase I isoform epsilon antibody
    • CKI epsilon antibody
    • CKI-epsilon antibody
    • CKIe antibody
    • CKIepsilon antibody
    • CSNK 1E antibody
    • CSNK1E antibody
    • DBT antibody
    • Doubletime, Drosophila, homolog of antibody
    • EC 2.7.11.1 antibody
    • epsilon isoform antibody
    • HCK IE antibody
    • KC1E antibody
    • KC1E_HUMAN antibody
    • kinase CK1 epsilon antibody
    • MGC 10398 antibody
    see all

Anti-CK1 epsilon antibody - Carboxyterminal end images

  • Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687) at 1/100 dilution + SKBR3 cell lysate at 12.5 µg

    Secondary
    HRP anti rabbit

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 100 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Formalin fixed and paraffin embedded human breast carcinoma tissue reacted with 1/50 ab71687. A peroxidase conjugated secondary antibody was then used, followed by DAB staining.

References for Anti-CK1 epsilon antibody - Carboxyterminal end (ab71687)

ab71687 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71687.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"