The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionBinds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
Tissue specificityFound in most tissues.
Sequence similaritiesBelongs to the TBCB family. Contains 1 CAP-Gly domain.
Post-translational modificationsPhosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR. Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.