Overview

  • Product nameAnti-CKAP1 antibody
    See all CKAP1 primary antibodies
  • Description
    Rabbit polyclonal to CKAP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-213 of Human CKAP1 (NP_001272).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates IHC-P: H1299 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab96101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionBinds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.
  • Tissue specificityFound in most tissues.
  • Sequence similaritiesBelongs to the TBCB family.
    Contains 1 CAP-Gly domain.
  • Post-translational
    modifications
    Phosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone.
  • Information by UniProt
  • Database links
  • Alternative names
    • CG22 antibody
    • CKAPI antibody
    • Cytoskeleton associated protein 1 antibody
    • Cytoskeleton associated protein CKAPI antibody
    • Cytoskeleton-associated protein 1 antibody
    • Cytoskeleton-associated protein CKAPI antibody
    • Tbcb antibody
    • TBCB_HUMAN antibody
    • Tubulin folding cofactor B antibody
    • Tubulin specific chaperone B antibody
    • Tubulin-folding cofactor B antibody
    • Tubulin-specific chaperone B antibody
    see all

Anti-CKAP1 antibody images

  • All lanes : Anti-CKAP1 antibody (ab96101) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa
  • ab96101, at a 1/500 dilution, staining CKAP1 in paraffin embedded H1299 xenograft by Immunohistochemical analysis.

References for Anti-CKAP1 antibody (ab96101)

ab96101 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"