Overview

  • Product nameAnti-CKAP2 antibody
    See all CKAP2 primary antibodies
  • Description
    Rabbit polyclonal to CKAP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Guinea pig, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 349-398 (GKAIVDSRSA QPKETSEERK ARLSEWKAGK GRVLKRPPNS VVTQHEPAGQ) of Human CKAP2 (NP_001091995).

  • Positive control
    • Human fetal lung lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab116460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPossesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
  • Tissue specificityAbundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
  • Sequence similaritiesBelongs to the CKAP2 family.
  • Developmental stagePresent at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI461656 antibody
    • AW228814 antibody
    • CKAP 2 antibody
    • Ckap2 antibody
    • CKAP2_HUMAN antibody
    • CTCL tumor antigen se20 10 antibody
    • CTCL tumor antigen se20-10 antibody
    • Cytoskeleton associated protein 2 antibody
    • Cytoskeleton-associated protein 2 antibody
    • DKFZp686L1238 antibody
    • FLJ10749 antibody
    • LB1 antibody
    • se20 10 antibody
    • TMAP antibody
    • Tumor and microtubule associated protein antibody
    • Tumor associated microtubule associated protein antibody
    • Tumor- and microtubule-associated protein antibody
    see all

Anti-CKAP2 antibody images

  • Anti-CKAP2 antibody (ab116460) at 1 µg/ml + Human fetal lung lysate at 10 µg

    Predicted band size : 77 kDa

References for Anti-CKAP2 antibody (ab116460)

ab116460 has not yet been referenced specifically in any publications.

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