Overview

  • Product name
  • Description
    Rabbit polyclonal to CKAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CKAP2 (internal sequence). The exact sequence is proprietary.
    Database link: Q8WWK9

  • Positive control
    • WB: Raji and NCI-H929 whole cell extracts. ICC/IF: HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
  • Tissue specificity
    Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
  • Sequence similarities
    Belongs to the CKAP2 family.
  • Developmental stage
    Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI461656 antibody
    • AW228814 antibody
    • CKAP 2 antibody
    • Ckap2 antibody
    • CKAP2_HUMAN antibody
    • CTCL tumor antigen se20 10 antibody
    • CTCL tumor antigen se20-10 antibody
    • Cytoskeleton associated protein 2 antibody
    • Cytoskeleton-associated protein 2 antibody
    • DKFZp686L1238 antibody
    • FLJ10749 antibody
    • LB1 antibody
    • se20 10 antibody
    • TMAP antibody
    • Tumor and microtubule associated protein antibody
    • Tumor associated microtubule associated protein antibody
    • Tumor- and microtubule-associated protein antibody
    see all

Images

  • All lanes : Anti-CKAP2 antibody (ab227214) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 2 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 77 kDa



    7.5% SDS-PAGE gel.

  • Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CKAP2 (green) using ab227214 at 1/500 dilution in ICC/IF.

    Nuclear Counterstain: Hoechst 33342 (blue).

References

ab227214 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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