Anti-CKAP2/LB1 antibody (ab85889)
Key features and details
- Rabbit polyclonal to CKAP2/LB1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CKAP2/LB1 antibody
See all CKAP2/LB1 primary antibodies -
Description
Rabbit polyclonal to CKAP2/LB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CKAP2/LB1 aa 633-683.
Database link: NP_001091995.1 -
Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab85889 was affinity purified using an epitope specific to CKAP2/LB1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner. -
Tissue specificity
Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney. -
Sequence similarities
Belongs to the CKAP2 family. -
Developmental stage
Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C). -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules. - Information by UniProt
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Database links
- Entrez Gene: 26586 Human
- Omim: 611569 Human
- SwissProt: Q8WWK9 Human
- Unigene: 444028 Human
- Unigene: 521482 Human
- Unigene: 728722 Human
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Alternative names
- AI461656 antibody
- AW228814 antibody
- CKAP 2 antibody
see all
Images
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All lanes : Anti-CKAP2/LB1 antibody (ab85889) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 90 seconds -
Detection of CKAP2/LB1 by Western Blot of Immunprecipitate.
ab85889, at 1 µg/ml, staining CKAP2/LB1 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 90 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab85889 has not yet been referenced specifically in any publications.