FunctionPossesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
Tissue specificityAbundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
Sequence similaritiesBelongs to the CKAP2 family.
Developmental stagePresent at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
Tumor associated microtubule associated protein antibody
Tumor- and microtubule-associated protein antibody
Anti-CKAP2 antibody images
Western blot - CKAP2 antibody (ab85889)
All lanes : Anti-CKAP2 antibody (ab85889) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
developed using the ECL technique
Predicted band size : 77 kDa Observed band size : 77 kDa
Exposure time : 90 seconds
Immunoprecipitation - CKAP2 antibody (ab85889)
Detection of CKAP2 by Western Blot of Immunprecipitate.
ab85889, at 1 µg/ml, staining CKAP2 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 90 seconds.
References for Anti-CKAP2 antibody (ab85889)
has not yet been referenced specifically in any publications.
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