Overview

  • Product name
  • Description
    Rabbit polyclonal to CKAP2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 633 and 683 of human CKAP2 (NP_001091995.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab85889 was affinity purified using an epitope specific to CKAP2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner.
  • Tissue specificity
    Abundant in testis, thymus, and in tumor derived cell lines, while barely detectable in liver, prostate, and kidney.
  • Sequence similarities
    Belongs to the CKAP2 family.
  • Developmental stage
    Present at the G1/S boundary. Accumulates as cells progress from S to G2 into mitosis. Rapidly degraded during mitosis exit by CDH1-activated anaphase promoting complex/cyclosome (APC/C).
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Contrary to the ectopically expressed protein, endogenous CKAP2 does not colocalize with microtubules in G1, S and early G2. At late G2 and prophase after separation of duplicated centrosomes, colocalizes with gamma-tubulin and centrosome-proximal microtubules. From prometaphase through anaphase B, colocalizes with mitotic spindle poles and spindle microtubules. During cytokinesis, absent from midbody microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI461656 antibody
    • AW228814 antibody
    • CKAP 2 antibody
    • Ckap2 antibody
    • CKAP2_HUMAN antibody
    • CTCL tumor antigen se20 10 antibody
    • CTCL tumor antigen se20-10 antibody
    • Cytoskeleton associated protein 2 antibody
    • Cytoskeleton-associated protein 2 antibody
    • DKFZp686L1238 antibody
    • FLJ10749 antibody
    • LB1 antibody
    • se20 10 antibody
    • TMAP antibody
    • Tumor and microtubule associated protein antibody
    • Tumor associated microtubule associated protein antibody
    • Tumor- and microtubule-associated protein antibody
    see all

Images

  • All lanes : Anti-CKAP2 antibody (ab85889) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 77 kDa


    Exposure time : 90 seconds
  • Detection of CKAP2 by Western Blot of Immunprecipitate.
    ab85889, at 1 µg/ml, staining CKAP2 in HeLa whole cell lysate immunoprecipitated using ab85889 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 90 seconds.

References

ab85889 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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