Overview

  • Product nameAnti-CKAP4 antibody
    See all CKAP4 primary antibodies
  • Description
    Rabbit polyclonal to CKAP4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 553-602 (LRTAVDSLVA YSVKIETNEN NLESAKGLLD DLRNDLDRLF VKVEKIHEKV) of Human CKAP4 (NP_006816).

  • Positive control
    • 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-CKAP4 antibody images

  • Anti-CKAP4 antibody (ab84705) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa

References for Anti-CKAP4 antibody (ab84705)

This product has been referenced in:
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - other (293T)
Specification 293T
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted Mar 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"