Overview

  • Product nameAnti-CKII alpha antibody [EP1963Y]
    See all CKII alpha primary antibodies
  • Description
    Rabbit monoclonal [EP1963Y] to CKII alpha
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CKII alpha aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P68400
    (Peptide available as ab203372)

  • Positive control
    • WB: HeLa cell lysate IHC-P Human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with CKII alpha peptide (ab203372).
Flow Cyt 1/20 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended.

  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Casein kinase 2 alpha 1 polypeptide antibody
      • Casein kinase II alpha 1 antibody
      • Casein kinase II alpha 1 subunit antibody
      • Casein kinase II alpha subunit antibody
      • Casein kinase II subunit alpha antibody
      • CK II alpha antibody
      • CK II antibody
      • CK2 alpha antibody
      • CK2 catalytic subunit alpha antibody
      • CK2A1 antibody
      • CKII antibody
      • CKIIalpha antibody
      • CSK21_HUMAN antibody
      • CSNK2A1 antibody
      see all

    Anti-CKII alpha antibody [EP1963Y] images



    • Predicted band size : 45 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CKII alpha knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green – ab76040 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab76040 was shown to specifically react with CKII alpha when CKII alpha knockout samples were used. Wild-type and CKII alpha knockout samples were subjected to SDS-PAGE. ab76040 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemical analysis of CKII alpha in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.
    • Anti-CKII alpha antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa
    • Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76040, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    References for Anti-CKII alpha antibody [EP1963Y] (ab76040)

    This product has been referenced in:
    • Manni S  et al. Protein Kinase CK2 Inhibition Down Modulates the NF-?B and STAT3 Survival Pathways, Enhances the Cellular Proteotoxic Stress and Synergistically Boosts the Cytotoxic Effect of Bortezomib on Multiple Myeloma and Mantle Cell Lymphoma Cells. PLoS One 8:e75280 (2013). WB, IHC-P ; Human . Read more (PubMed: 24086494) »

    See 1 Publication for this product

    Product Wall

    Thank you for your phone call and for your patience while I have been in touch with the lab about ab76040. The immunogen of the antibody shares 64% identity with murine CK II alpha prime protein, so cross-reactivity is not predicted. However, the c...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"