Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410)
Key features and details
- Rabbit polyclonal to CSNK2A1 (phospho T360 + S362)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-CSNK2A1 (phospho T360 + S362) antibody
See all CSNK2A1 primary antibodies -
Description
Rabbit polyclonal to CSNK2A1 (phospho T360 + S362) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human CSNK2A1 (phospho T360 + S362) conjugated to keyhole limpet haemocyanin.
Database link: NP_001894.2 -
Positive control
- Recombinant proteins CSNK2A1 wt, CSNK2A1 T360A, CSNK2A1 S362A, CSNK2A1T360/S362A phoshorylated by ERK2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab119410 was purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Alternative names
- Casein kinase 2 alpha 1 polypeptide antibody
- Casein kinase 2 alpha antibody
- Casein kinase II alpha 1 antibody
see all
Images
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All lanes : Anti-CSNK2A1 (phospho T360 + S362) antibody (ab119410) at 1/1000 dilution
Lane 1 : CSNK2A1
Lane 2 : CSNK2A1 + ERK2
Lane 3 : CSNK2A1 T360A
Lane 4 : CSNK2A1 T360A + ERK2
Lane 5 : CSNK2A1 S362A
Lane 6 : CSNK2A1 S362A + ERK2
Lane 7 : CSNK2A1 T360/S362A
Lane 8 : CSNK2A1 T360/S362A + ERK2
Predicted band size: 45 kDaLower panel stained with CSNK2A1 antibody as a loading control.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab119410 has been referenced in 3 publications.
- Kim JE et al. Src/CK2/PTEN-Mediated GluN2B and CREB Dephosphorylations Regulate the Responsiveness to AMPA Receptor Antagonists in Chronic Epilepsy Rats. Int J Mol Sci 21:N/A (2020). PubMed: 33348808
- Ali R et al. ATM Regulated PTEN Degradation Is XIAP E3 Ubiquitin Ligase Mediated in p85a Deficient Cancer Cells and Influence Platinum Sensitivity. Cells 8:N/A (2019). PubMed: 31635307
- Li X et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930