The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceCklfsf8 belongs to the chemokine-like factor superfamily, a novel superfamily that is similar to the chemokine family and the transmembrane 4 superfamily. Cklfsf8 is one of several chemokine-like factors; it is widely expressed in many tissues, but it's the exact function is unknown.