Overview

  • Product nameAnti-CKMT2 antibody
    See all CKMT2 primary antibodies
  • Description
    Rabbit polyclonal to CKMT2
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    A region within synthetic peptide: VCAEVREQPR LFPPSADYPD LRKHNNCMAE CLTPAIYAKL RNKVTPNGYT, corresponding to N terminal amino acids 37-86 of Human CKMT2

  • Positive control
    • Jurkat cell lysate Human skeletal muscle tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab55963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-CKMT2 antibody images

  • Anti-CKMT2 antibody (ab55963) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffine-embedded sections) analysis of human muscle tissue labelling CKMT2 with ab55963 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

References for Anti-CKMT2 antibody (ab55963)

This product has been referenced in:
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). WB ; Human . Read more (PubMed: 21242281) »
  • Bansal A  et al. Proteomic analysis reveals late exercise effects on cardiac remodeling following myocardial infarction. J Proteomics 73:2041-9 (2010). WB ; Rat . Read more (PubMed: 20601275) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brown adipose tissue)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 8 µg
Treatment by a drug which is unknown whether it stimulates or inhibits CKMT2
Specification brown adipose tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Apr 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"