Overview

  • Product nameAnti-CKS1/CKS2 antibody
  • Description
    Mouse monoclonal to CKS1/CKS2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: MSHKQIYYSD KYDDEEFEYR HVMLPKDIAK LVPKTHLMSE SEWRNLGVQQ SQGWVHYMIH EPEPHILLFR RPLPKKPKK, corresponding to amino acids 1-80 of Human CKS1/CKS2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCks (Cdc kinase subunit ) proteins are members of the cyclin dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human non small cell lung carcinoma.
    • Database links
    • Alternative names
      • CDC28 protein kinase 2 antibody
      • CDC28 protein kinase regulatory subunit 1B antibody
      • CDC28 protein kinase regulatory subunit 2 antibody
      • CKS1 antibody
      • CKS1B antibody
      • CKS2 antibody
      • CKSHS2 antibody
      • Cyclin-dependent kinases regulatory subunit 1 antibody
      see all

    Anti-CKS1/CKS2 antibody images

    • Western blot against tagged recombinant protein immunogen using ab54643 CKS1/CKS2 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-CKS1/CKS2 antibody (ab54643)

    ab54643 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab54643.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"