The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
RelevanceCks (Cdc kinase subunit ) proteins are members of the cyclin dependent kinase (cdk) family that act as adaptors to allow the cdks to interact more efficiently with their molecular targets. Cks1 is involved in the regulation of cell cycle mitosis in normal and cancer cells. Cks1 promotes mitosis by modulating the transcriptional activation of the APC/C protein-ubiquitin ligase activator Cdc20. Cks1 has also been established as an essential cofactor in the ubiquitylation of Cdk inhibitor p27 by Skp2 ubiquitin ligase. Mammalian Cks1 binds to Skp2 and promotes the association of Skp2 with phosphorylated p27 (Thr187), which further induces ubiquitylation of p27. High expression of Cks1 may be involved in the pathogenesis of human non small cell lung carcinoma.