Overview

  • Product nameAnti-CLASP1 antibody
    See all CLASP1 primary antibodies
  • Description
    Rabbit polyclonal to CLASP1
  • SpecificityDetects endogenous levels of total CLASP1 protein
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from internal region of human CLASP1

  • Positive control
    • Extracts from Jurkat cells (WB); human testis tissue (IHC-P)

Properties

Applications

Our Abpromise guarantee covers the use of ab110010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 169 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionMicrotubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
  • Sequence similaritiesBelongs to the CLASP family.
    Contains 7 HEAT repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700030C23Rik antibody
    • 5730583A19Rik antibody
    • B130045P17Rik antibody
    • CLAP1_HUMAN antibody
    • clasp1 antibody
    • CLIP associating protein 1 antibody
    • CLIP associating protein CLASP1 antibody
    • CLIP-associating protein 1 antibody
    • Cytoplasmic linker associated protein 1 antibody
    • Cytoplasmic linker-associated protein 1 antibody
    • DKFZp686D1968 antibody
    • DKFZp686H2039 antibody
    • FLJ33821 antibody
    • FLJ41222 antibody
    • hOrbit1 antibody
    • KIAA0622 antibody
    • MAST1 antibody
    • MGC131895 antibody
    • mKIAA0622 antibody
    • Multiple asters 1 antibody
    • Multiple asters homolog 1 antibody
    • Protein Orbit homolog 1 antibody
    see all

Anti-CLASP1 antibody images

  • All lanes : Anti-CLASP1 antibody (ab110010) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells (5-30 ug total protein) with no immunizing peptide
    Lane 2 : extracts from Jurkat cells (5-30 ug total protein) with immunizing peptide (5-10 ug)


    Predicted band size : 169 kDa
  • Immunohistochemistry analysis of paraffin embedded human testis tissue, using ab110010 at 1/50. The picture on the right is treated with the immunizing peptide.

References for Anti-CLASP1 antibody (ab110010)

ab110010 has not yet been referenced specifically in any publications.

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