Overview

  • Product name
  • Description
    Rabbit polyclonal to CLASP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1-179 of Human CLASP1 (Q7Z460).

  • Positive control
    • HepG2 and mouse brain whole cell lysates; 4T1 xenograft tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
  • Sequence similarities
    Belongs to the CLASP family.
    Contains 7 HEAT repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700030C23Rik antibody
    • 5730583A19Rik antibody
    • B130045P17Rik antibody
    • CLAP1_HUMAN antibody
    • clasp1 antibody
    • CLIP associating protein 1 antibody
    • CLIP associating protein CLASP1 antibody
    • CLIP-associating protein 1 antibody
    • Cytoplasmic linker associated protein 1 antibody
    • Cytoplasmic linker-associated protein 1 antibody
    • DKFZp686D1968 antibody
    • DKFZp686H2039 antibody
    • FLJ33821 antibody
    • FLJ41222 antibody
    • hOrbit1 antibody
    • KIAA0622 antibody
    • MAST1 antibody
    • MGC131895 antibody
    • mKIAA0622 antibody
    • Multiple asters 1 antibody
    • Multiple asters homolog 1 antibody
    • Protein Orbit homolog 1 antibody
    see all

Images

  • Anti-CLASP1 antibody (ab154087) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg

    Predicted band size: 16 kDa



    5% SDS PAGE
  • Anti-CLASP1 antibody (ab154087) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 16 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded 4T1 xenograft tissue labeling CLASP1 with ab154087 at 1/100 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling CLASP1 with ab154087 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.

References

ab154087 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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