Overview

  • Product nameAnti-CLASP2 antibody
    See all CLASP2 primary antibodies
  • Description
    Rabbit polyclonal to CLASP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the Y1019 region of Human CLASP2 (UniProt ID O75122).

  • Positive control
    • Mouse spleen tissue lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95005 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab95005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 141 kDa.

Target

  • FunctionMicrotubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.
  • Tissue specificityBrain-specific.
  • Sequence similaritiesBelongs to the CLASP family.
    Contains 8 HEAT repeats.
  • DomainThe two SXIP sequence motifs mediate interaction with MAPRE1; this is necessary for targeting to growing microtubule plus ends.
    Two TOG regions display structural characteristics similar to HEAT repeat domains and mediate interaction with microtubules.
  • Post-translational
    modifications
    Phosphorylated by GSK3B. Phosphorylation reduces MAPRE1 binding (PubMed:26003921). Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella.
  • Cellular localizationCytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Golgi apparatus, trans-Golgi network. Cell membrane. Cell projection, ruffle membrane. Localizes to microtubule plus ends (PubMed:15631994). Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase (PubMed:16866869, PubMed:16914514). In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2 (PubMed:16824950). The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLAP2_HUMAN antibody
    • CLASP 2 antibody
    • Clasp2 antibody
    • CLIP associating protein 2 antibody
    • CLIP-associating protein 2 antibody
    • Cytoplasmic linker associated protein 2 antibody
    • Cytoplasmic linker-associated protein 2 antibody
    • hOrbit2 antibody
    • KIAA0627 antibody
    • Multiple asters (Mast) like homolog 2 antibody
    • Protein Orbit homolog 2 antibody
    see all

Anti-CLASP2 antibody images

  • Anti-CLASP2 antibody (ab95005) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 141 kDa

References for Anti-CLASP2 antibody (ab95005)

ab95005 has not yet been referenced specifically in any publications.

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