Overview

  • Product nameAnti-Claspin antibodySee all Claspin primary antibodies ...
  • Description
    Rabbit polyclonal to Claspin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Claspin encoded within exon 8.

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000 - 1/5000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa).

Target

  • FunctionRequired for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
  • Sequence similaritiesBelongs to the claspin family.
  • DomainThe C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
  • Post-translational
    modifications
    Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q9HAW4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Claspin antibody
    • claspin homolog (Xenopus laevis) antibody
    • Claspin homolog antibody
    • Clspn antibody
    • CLSPN protein antibody
    • CLSPN_HUMAN antibody
    • hClaspin antibody
    • Hu Claspin antibody
    see all

Anti-Claspin antibody images



  • Predicted band size : 180 kDa


    Sample: Hela cell lysate (20µg)

    Antibody: Affinity purified Rabbit anti-Claspin (ab3719) used at a 1 / 1000 and 1 / 2000 dilution with (+) or without (-) blocking peptide.

References for Anti-Claspin antibody (ab3719)

This product has been referenced in:
  • Jiang K  et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 278:25207-17 (2003). Read more (PubMed: 12676962) »

See 1 Publication for this product

Product Wall

The immunogen is from residues 450 and 500 of human Claspin (GeneID 63967).

Unfortunately we are not able to provide the blocking peptide for this antibody.

Residues 336 to 526 of human claspin (AF297866_1) are encoded by exon 8. For information on all the exons of Human Claspin, go to http://www.ncbi.nlm.nih.gov/sutils/evv.cgi?contig=NT_079482.1&gene=CLSPN&lid=63967 For information on the exons of o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"