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Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)
![Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)](/ps/datasheet/images/ab2731_1.jpg)
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Western blot - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)
![Western blot - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)](/ps/datasheet/images/2/ab2731/Clathrin-Primary-antibodies-ab2731-2.jpg)
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Product Name
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Clathrin antibody [X22] - Membrane Vesicle Marker
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See all Clathrin - Membrane Vesicle Marker antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [X22] to Clathrin - Membrane Vesicle Marker
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Immunogen
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Purified human brain clathrin heavy chain
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Reacts with
(species key)
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Hu, Ms, Rat, Cow
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Specificity
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Detects clathrin heavy chain.
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Epitope
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Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
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Tested applications
(see key)
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ICC/IF, IM, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IF: 1/1000.
IM: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Immunoprecipitation of triskelions with this antibody allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.
WB: 1/500. Detects a band of approximately 180 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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bovine brain extract
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Cellular localization
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Cytoplasmic face of coated pits and vesicles.
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Research areas
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Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
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Relevance
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Membrane vesicle formation is a process required for the endocytosis and biosynthesis of various secreted and membrane bound proteins. Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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X22
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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6.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Clathrin antibody [X22] - Membrane Vesicle Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Clathrin antibody [X22] - Membrane Vesicle Marker images, references, products related to ab2731 and other tools.
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Clathrin antibody [X22] - Membrane Vesicle Marker images:
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 Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)
ab2731 staining human HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.01% Triton X-100 in PBS prior to blocking in 1% BSA for 1 hour at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 1 hour at 22°C. An Alexa Fluor® 405 conjugated goat anti-mouse antibody diluted 1/400 was used as the secondary. This image is courtesy of an Abreview submitted by Mrs Danijela Markovic
See Abreview
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Western blot - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)
All lanes : Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731) at 1/500 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Additional bands at : 240 kDa,450 kDa. We are unsure as to the identity of these extra bands.
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References for Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)
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This product has been used in: (two most recent references)
Wlchli S et al. Characterization of clathrin and Syk interaction upon Shiga toxin binding. Cell Signal 21:1161-8 (2009). PubMed: 19289168
Teckchandani A et al. Quantitative proteomics identifies a Dab2/integrin module regulating cell migration. J Cell Biol 186:99-111 (2009). ICC/IF; Human. PubMed: 19581412
See all 16 publication references for this product.
If you publish research using ab2731 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Clathrin
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Clathrin antibody [X22] - Membrane Vesicle Marker - more information
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Clathrin antibody [X22] - Membrane Vesicle Marker - related products:
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Clathrin antibody [X22] - Membrane Vesicle Marker - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)](/index.html?datasheet=2731#ab2731_1.jpg)
![Western blot - Clathrin antibody [X22] - Membrane Vesicle Marker (ab2731)](/index.html?datasheet=2731#Clathrin-Primary-antibodies-ab2731-2.jpg)
