SpecificityRecognizes the clathrin heavy chain from a diverse range of species. In nervous tissue it crossreacts with another coated vesicle polypeptide of 190kD which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may crossreact with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent concentration. Predicted molecular weight: 192 kDa.
FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
Sequence similaritiesBelongs to the clathrin heavy chain family.
Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
References for Anti-Clathrin heavy chain antibody [2Q2166] (ab14401)
This product has been referenced in:
Sens-Abuázar C et al. Down-regulation of ANAPC13 and CLTCL1: Early Events in the Progression of Preinvasive Ductal Carcinoma of the Breast. Transl Oncol5:113-23 (2012).
Read more (PubMed: 22496928) »
Marx C et al. ErbB2 trafficking and degradation associated with K48 and K63 polyubiquitination. Cancer Res70:3709-17 (2010).
Read more (PubMed: 20406983) »