Overview

  • Product name
    Anti-Clathrin light chain antibody
    See all Clathrin light chain primary antibodies
  • Description
    Goat polyclonal to Clathrin light chain
  • Specificity
    Expected to recognize all reported isoforms of light chains a and b.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
  • Immunogen

    Synthetic peptide:

    CDFNPKSSKQAKD

    from Human Clathrin light chain(NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1)

  • Positive control
    • Human frontal cortex lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg

      Predicted band size : 23 kDa
      Observed band size : 23 kDa
      Additional bands at : 35 kDa (possible isoform).

    References

    This product has been referenced in:
    • Liu H  et al. Clathrin-mediated endocytosis in living host cells visualized through quantum dot labeling of infectious hematopoietic necrosis virus. J Virol 85:6252-62 (2011). WB . Read more (PubMed: 21525360) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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