Anti-Clathrin light chain antibody [CON.1] (ab24579)


  • Product nameAnti-Clathrin light chain antibody [CON.1]
    See all Clathrin light chain primary antibodies
  • Description
    Mouse monoclonal [CON.1] to Clathrin light chain
  • Specificityab24579 recognises Clathrin Light Chain.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammal
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 23/44 of Cow Clathrin light chain



Our Abpromise guarantee covers the use of ab24579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. (see Abreview).
WB 1/250. Predicted molecular weight: 25-29 kDa.
ICC/IF Use at an assay dependent concentration.
IP 1/200.


Anti-Clathrin light chain antibody [CON.1] images

  • Formalin fixed HeLa cells double stained with the monoclonal antibody to clathrin, (ab24579, Red) and giantin (green). The nucleus was visualized with DAPI stain.
  • ab74579 staining Clatherin light chain in human prostate carcinoma tissue by immunohistochemistry (IHC-P - formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 1 hour at 21 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody 1/1000 (PBS) for 1 hour at 21 °C. A Biotin-conjugated Donkey polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody.

    See Abreview

References for Anti-Clathrin light chain antibody [CON.1] (ab24579)

This product has been referenced in:
  • Poulsen ET  et al. New Insights to Clathrin and Adaptor Protein 2 for the Design and Development of Therapeutic Strategies. Int J Mol Sci 16:29446-53 (2015). IP . Read more (PubMed: 26690411) »
  • Campos SK  et al. Opposing Effects of Bacitracin on Human Papillomavirus Type 16 Infection: Enhancement of Binding and Entry and Inhibition of Endosomal Penetration. J Virol : (2012). Read more (PubMed: 22345461) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam.

Unfortunately we do not have any western blot images that I can send you, the 1/250 dilution is the recommendation that we received from our sourcing lab.

Although it does seem high, I would recommend...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate Carcinoma)
Specification Prostate Carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Aug 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (neuronal stem cells)
Specification neuronal stem cells
Fixative Paraformaldehyde
Permeabilization Yes - 0,5% Saponin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Dr. Dominik Schelshorn

Verified customer

Submitted Feb 05 2008

Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I have read your questionnaire and I have a few comments. Please can you tell me the molecu...

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