Overview

  • Product nameAnti-Claudin 1 antibody [1C5-D9]
    See all Claudin 1 primary antibodies
  • Description
    Mouse monoclonal [1C5-D9] to Claudin 1
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-212 of Human Claudin 1 (AAH12471) with GST tag (MW of the GST tag alone is 26 kDa).

  • Positive control
    • Human breast tissue; Human pancreas tissue; Human uterus tissue PRC/PRF/5 lysate; A431 lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number1C5-D9
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

Target

  • FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody [1C5-D9] images

  • Human breast (formalin fixed, paraffin embedded) stained with ab115225 at 5 ug/ml.
  • Human pancreas (formalin fixed, paraffin embedded) stained with ab115225 at 5 ug/ml.
  • Human uterus (formalin fixed, paraffin embedded) stained with ab115225 at 5 ug/ml.


  • Predicted band size : 23 kDa


  • Predicted band size : 23 kDa

References for Anti-Claudin 1 antibody [1C5-D9] (ab115225)

This product has been referenced in:
  • Sung PS  et al. Hepatitis C virus entry is impaired by claudin-1 downregulation in diacylglycerol acyltransferase-1-deficient cells. J Virol 88:9233-44 (2014). Read more (PubMed: 24899196) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"