Overview

  • Product nameAnti-Claudin 1 antibody
    See all Claudin 1 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 1
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, IP, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog, Monkey
  • Immunogen

    Synthetic peptide (Human) (C terminal).

  • Positive control
    • Tested with skin and breast carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IP 1/2500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21719782
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 19284967

Target

  • FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similaritiesBelongs to the claudin family.
  • Cellular localizationCell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody images

  • ab15098 (1µg/ml) staining Claudin 1 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab15098 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15098, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15098 staining Claudin 1 in mouse kidney tissue sections by IHC-P (paraformaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 20% serum for 30 minutes at 22°C; antigen retrival was by heat mediation in Citrate buffer (pH6). The sample was incubated with primary antibody (1/500 in blocking buffer) at 22°C for 16 hours. A Biotin-conjugated Donkey polyclonal to rabbit IgG (1/250) was used as secondary antibody.

    See Abreview

  • Human skin stained with ab15098.
  • Anti-Claudin 1 antibody (ab15098) at 1/25 dilution + HeLa cell lysate

    Predicted band size : 23 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of acetone-fixed frozen murine mammary gland, labelling Claudin 1 with ab15098 used at a dilution of 1/200 incubated for 16 hours at 4°C in PBS with 0.025% Triton. Blocking was with 10% serum incubated for 1 hour at 25°C. The secondary used was a polyclonal goat anti-rabbit Alexa Fluor® 568 conjugate used at 1/1000.

    See Abreview

References for Anti-Claudin 1 antibody (ab15098)

This product has been referenced in:
  • Kyoko OO  et al. Expressions of tight junction proteins Occludin and Claudin-1 are under the circadian control in the mouse large intestine: implications in intestinal permeability and susceptibility to colitis. PLoS One 9:e98016 (2014). WB ; Mouse . Read more (PubMed: 24845399) »
  • Süren D  et al. Loss of tight junction proteins (Claudin 1, 4, and 7) correlates with aggressive behavior in colorectal carcinoma. Med Sci Monit 20:1255-62 (2014). IHC-P ; Human . Read more (PubMed: 25038829) »

See all 16 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Jejunum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH for 20 min and then Low pH citrate buffer
Permeabilization Yes - 0.1% Triton-X-100
Specification Jejunum
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Z-FIX
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Submitted Oct 10 2016

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Application Western blot
Sample Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 06 2016

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 14 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 24 2016

Abreviews
Application Western blot
Sample Pig Tissue lysate - whole (Intestinal tissue (mucosa))
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 25 µg
Specification Intestinal tissue (mucosa)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 17 2016

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Application Western blot
Sample Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 20 µg
Specification Intestinal tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 17 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Skin)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Protease type XIV (Sigma P5147)
Permeabilization No
Specification Skin
Blocking step BioGenex Power Block 10 minutes, then BioGenex Protein Block (NGS) 20 minutes as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative Formaldehyde
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Submitted Nov 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
Permeabilization No
Specification skin
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
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Submitted Oct 16 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse Mammary Gland)
Specification Mouse Mammary Gland
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Acetone
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Submitted Aug 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Nerve Bundle adjacent to Temporal Artery Biopsy)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization No
Specification Nerve Bundle adjacent to Temporal Artery Biopsy
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
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Submitted May 15 2015

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney)
Specification kidney
Fixative Methanol
Permeabilization Yes - 1% Triton X
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Nov 07 2012

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"