The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 23 kDa.
Use a concentration of 3 µg/ml.
FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
Sequence similaritiesBelongs to the claudin family.
Claudin 1 antibody (ab56417) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - Claudin 1 antibody (ab56417)
Predicted band size : 23 kDa Claudin 1 antibody (ab56417) at 1ug/lane + PRC/PRF/5 cell lysate at 25ug/lane.
References for Anti-Claudin 1 antibody (ab56417)
This product has been referenced in:
Takahashi S et al. Expression of adhesion molecules and epithelial-mesenchymal transition factors in medullary carcinoma of the colorectum. Hum Pathol46:1257-66 (2015).
Read more (PubMed: 26208847) »
Koda R et al. Expression of tight junction protein claudin-1 in human crescentic glomerulonephritis. Int J Nephrol2014:598670 (2014).
Read more (PubMed: 24868462) »