Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 1
  • Specificity
    Detects endogenous levels of total Claudin 1 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non phosphopeptide (Human) from around the phosphorylation site of tyrosine 210 (GKDYPV)

  • Positive control
    • HeLa cells, treated with Hu (2nM, 24hours)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
ELISA 1/20000.

Target

  • Function
    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificity
    Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in disease
    Ichthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Anti-Claudin 1 antibody images

  • All lanes : Anti-Claudin 1 antibody (ab79404) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with Hu (2nM, 24hours)
    Lane 2 : HeLa cell extract treated with Hu (2nM, 24hours) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-Claudin 1 antibody (ab79404)

ab79404 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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